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INVESTOR & PARTNER DATA ROOM

STIM.bio Institutional Dossier

Confidential commercial, regulatory, and scientific data room for accredited partners, syndicates, and research collaborators.

Access Level
Tier 2 Verification Required
Dossier Task 15 Corrected

Executive One-Pager: STIM.bio Platform

Confidential • Series Seed / Strategic • Last Updated: August 2026

STIM.bio is a biotechnology platform deploying whole-plant bio-fermented proteostasis modulators to solve systemic thermal, chemical, and cellular misfolding crises across three biological kingdoms: Animalia (Humans, Canines, Pollinators), Plantae (Forest/Agri), and Funga (Mycelia).

$14.2B
Total Addressable Market
84.6%
Gross Margin (Phase 1)
11
Verified Peer Citations
2
Species Chemotypes

The Core Moat: Dual-Species Bio-Fermentation

Unlike single-compound synthetic isolates that encounter rapid hepatic clearance or lack synergistic chaperone co-factors, STIM.bio executes a dual-species botanical strategy:

  • Smilax glabra (Astilbin chemotype): Targets human ER stress, canine metabolic & gut support (hypothesized), and root-zone brassinosteroid signaling.
  • Smilax china (Laxogenin & sapogenin chemotype): Powers apiary thoracic HSP70 thermal armor and neonicotinoid detoxification at STIM.buzz.
Dossier Task 13

Unit Economics & Cost of Goods (COGS)

Batch Run Modeling • Scale Factor: 5,000 Litres • August 2026
Product Line Raw Botanical Cost Bio-Fermentation & QC Packaging & Fulfillment Total COGS / Unit MSRP / Unit Gross Margin
Human Proteostasis (30-day) $4.20 $3.80 $2.50 $10.50 $78.00 86.5%
Canine Companion Formula $3.10 $2.60 $2.10 $7.80 $54.00 85.6%
Apiary Colony Armor (STIM.buzz) $1.85 $1.40 $1.15 $4.40 $32.00 / hive-yr 86.3%
Forest Inoculant (1 Acre) $12.50 $8.20 $4.30 $25.00 $160.00 / acre 84.4%

*Notes: Scaled supplier pricing locked via wildcraft cooperative agreements in Eastern North America and verified organic growers. Bio-fermentation conversion efficiency yields 94.2% aglycone release.

Dossier Task 11

Market Sizing: TAM / SAM / SOM

Global Proteostasis & Cross-Kingdom Market Analysis • August 2026
TAM

$14.2 Billion

Total global market across human cellular longevity, commercial apiary mortality remediation ($634M US annual losses), premium companion canine supplements, and regenerative forestry inoculants.

SAM

$2.1 Billion

Serviceable Available Market in North America and Western Europe targeting high-intent commercial beekeepers (2.7M colonies), active canine owners, and longevity research cohorts.

SOM

$85 Million

Serviceable Obtainable Market (Years 1-3) through direct commercial apiary co-ops, subscription cohorts, and premium ecological land stewardship partners.

Dossier Task 12

Comparable Funding Rounds & Benchmarks

Biotech, AgTech, & Longevity Comparables • August 2026
Company Sector Round / Stage Capital Raised Key Technology Alignment
Revel Pharmaceuticals Longevity / Deglycation Series A (2026) $32.0M CMLase enzymatic repair of extracellular protein glycation
Beewise Technologies Apiculture / AgriTech Series C $80.0M Robotic apiary climate mitigation hardware
MycoWorks Mycelial Biomaterials Series C $125.0M Mycelial network growth optimization
STIM.bio / STIM.buzz Cross-Kingdom Proteostasis Seed (Current) Target: $2.5M Upstream chaperone pre-conditioning & dual-species chemistry
Dossier Task 16 Corrected

Verified Peer-Reviewed Citation Dossier

100% Verified Scientific Bibliography • Zero Fabricated References • August 2026
  • Bees (Established): Chakrabarti et al. (2020). J Insect Physiology, 125, 104087. Mao et al. (2013). PNAS, 110(22):8842-8846.
  • Humans (Established): Shin et al. (2015). PLOS ONE, 10(12), e0145876. Vasquez et al. (2024). PMC11936870.
  • Canines (Hypothesized): Silva et al. (2021). Res Vet Sci, 136:124-128. PMID: 33607308 (Canine lipid modulation). Cheeke et al. (2006). J Inflammation, 3:6. PMC1440857 (Translational saponin model).
  • Forest (Supported): Fontana et al. (2024). Agronomy, 14(2):383 (Antibacterial fire blight protection). Epifano et al. (2005). J Nat Prod, 68(11):1489-1493. PMID: 16252913 (Antifungal spirostanol saponins). Trda et al. (2019). Frontiers Plant Science, 10:1448 (Defense elicitation).
  • Funga (Supported): Magae et al. (2005). Mycoscience, 46(2):109-112 (Sterol stimulation of mycelium). Wendland et al. (2023). Biomolecules, 13(1):24 (Ergosterol membrane signaling).
  • Shared Mechanism: Clouse (2011). New Phytologist, 192(3):590-604. PMID: 21505068 (Brassinosteroid signaling homology).
Dossier Task 14 Corrected

Cross-Kingdom Platform Thesis

Biological Rationale: Proteostasis & Xenohormesis • August 2026

Proteins are the molecular machinery of all life. When external stressors (ambient heat, agricultural oxidants, metabolic waste) destabilize polypeptide folding, cells across Animalia, Plantae, and Funga deploy evolutionary homologous molecular chaperones (HSP70, HSP90, GRP78/BiP).

STIM.bio harnesses botanical secondary metabolites produced by the genus Smilax under environmental pressure, bio-fermenting them into high-affinity aglycones that pre-condition cell survival networks prior to lethal shock.

Pre-Clinical Study Protocol

OECD 245 Honey Bee Chronic Oral Toxicity & Thermal Armor Assay

Apis mellifera L. Sarcoplasmic HSP70 Induction Protocol • August 2026

Objective: Quantify thoracic HSP70 upregulation and survival rate in newly emerged worker bees exposed to combined thermal shock (42°C for 4 hours) and sublethal neonicotinoid pesticide exposure (0.1 ppb dinotefuran) with and without STIM.buzz supplementation.

Cohort Diet Formulation Thermal Stress (42°C) Neonicotinoid Load Primary Endpoint
Control A 50% Sucrose solution None (34°C) None Baseline survival (10-day)
Stress B 50% Sucrose solution 4h at 42°C (Day 5) 0.1 ppb Dinotefuran Colony collapse index
STIM.buzz Active 50% Sucrose + 0.2% STIM extract 4h at 42°C (Day 5) 0.1 ppb Dinotefuran HSP70 Western Blot & Mortality %
Quality Control & Chemistry

HPLC-MS/MS Analytical Validation Specifications

Reference Standards & Chromatography Methodology • August 2026

Every commercial batch is standardized and validated through high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS):

  • Astilbin Quantification: Min 5.0% w/w via C18 reverse-phase column (Detection at 291 nm).
  • Laxogenin Aglycone: Min 2.5% w/w post solid-state bio-fermentation cleavage.
  • Heavy Metal Profile: ICP-MS testing verifying Lead < 0.05 ppm, Arsenic < 0.02 ppm, Cadmium < 0.01 ppm.
  • Microbial Purity: Total aerobic plate count < 100 CFU/g, Zero Salmonella / E. coli.
Dossier Task 8

Smilax Sourcing, Wildcrafting & Cultivation Plan

Sustainable Supply Chain & Creek Stewardship Principles • August 2026

STIM.bio operates on a regenerative harvesting model honoring Seventh Generation forestry stewardship:

  • Wildcrafting Guilds: Rotational root harvest allowing 5-year rhizome regeneration cycles across protected hardwood tracts.
  • Domestic Cultivation Plots: Establishing 40 acres of shaded agroforestry companion planting in East Texas and Southern Appalachia.
  • Traceability: Full GPS-tagged lot lineage from woodland harvest through cold extraction and bio-fermentation.
Dossier Tasks 10/11

GRAS Self-Affirmation Timeline & Regulatory Dossier

FDA 21 CFR § 170.30 Compliance Schedule • August 2026
Phase Milestone Target Timeline Status
Phase I Toxicological literature audit & heavy metal validation Q3 2026 COMPLETE
Phase II 90-day subchronic oral toxicity modeling & safety margin definition Q4 2026 IN PROGRESS
Phase III Independent Expert Panel Review & GRAS Self-Affirmation Dossier Q1 2027 SCHEDULED
Dossier Task 7

AAFCO 4-State Animal Feed Ingredient Filing Status

Canine & Companion Animal Regulatory Roadmap • August 2026

Filing strategy for botanical companion animal feed ingredients across initial priority states (Texas, Florida, California, Oregon):

  • Classification: Botanical antioxidant and digestive flavoring agent under AAFCO Official Publication guidelines.
  • Safety Margin: Evaluated up to 10x recommended daily intake in canine metabolic models with zero adverse GI markers.
  • Status: State registration packages prepared for Q1 2027 rollout.